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V5-Tag Monoclonal Antibody

货号:PGM3005
包装:100µg
价格:1500
储存:-20°C

详细描述

Catalog No.
PGM3005
Size
100ug
Source
Mouse
Immunogen
Synthetic Peptide
Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Specificity
The antibody detects C-terminal, internal, and N-terminal V5-tag fusion proteins.
Formulation
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Concentration
1 mg/ml
Storage / Stability
-20°C/1 year
Reactivity
N/A
Applications
WB, IP, IF
Dilution
WB: 1:5000 IP: 1:200 IF: 1:1000
V5-Tag Monoclonal Antibody Images
IP antibody use: 5ug V5 Mouse IgG1 per ml Lysate, WB 1: 5000 1) untransfected 293 cell lysate 2) transfected 293 cell lysate with V5-tag fusion protein 3) IP (untransfted 293+anti-V5 mAb+Protein G agarose) 4) IP (transfected 293+ normal Mouse IgG+Prote
IF analysis of 293 cells transfected with a V5-tag protein,1:2000 dilution (blue DAPI, red anti-V5)
1ug V5 fusion protein+ Primary antibody dilution at 1) 1:5000 2) 1:10000
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