-
CAS#
规格
单价
说明书
-
100ug
产品说明
Catalog No. PGM3005 Size 100ug Source Mouse Immunogen Synthetic Peptide Purification The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. Specificity The antibody detects C-terminal, internal, and N-terminal V5-tag fusion proteins. Formulation PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. Concen… 【产品详情】
产品说明
取200mg幼嫩叶片, 装入2ml离心管,加液氮研磨(加钢珠或玻璃珠在振荡器中处理5分钟);向上述研磨过的样品中,加植物组织抽提液100ul(如果加500mg叶片,则需要用250ul抽提液), 研磨2分钟(具体做法是:加钢珠或玻璃珠在振荡器中处理5分钟);12000rpm离10分钟,4℃;用移液器吸取上清(一般是80-120ul),转移至新的1.5ml离心管,做好标记;加入1/3体积的5x SDS loading buffer (for PAGE),混匀,100℃ 处理… 【产品详情】