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Immunogen
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Synthetic Peptide
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Purification
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The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
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Specificity
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The antibody detects C-terminal, internal, and N-terminal His-tag fusion proteins.
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Formulation
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PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
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Storage / Stability
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-20°C/1 year
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Dilution
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WB: 1:3000 IP: 1:200 IF: 1:1000
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His-Tag Monoclonal Antibody Images
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IP antibody use: 5ug His Mouse IgG1 per ml Lysate, WB 1: 3000 1) untransfected 293 cell lysate 2) transfected 293 cell lysate with His-tag fusion protein 3) IP (untransfected 293+anti-His mAb+ Protein G agarose) 4) IP (transfected 293+ normal Mouse IgG
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IF analysis of 293 cells transfected with a His-tag protein,1:1000 dilution (blue DAPI, red anti-His)
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2ug His fusion protein+ Primary antibody dilution at 1) 1:5000 2) 1:10000
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